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Laboratory Facilities at the Diabetes Unit



Our laboratory maintains global standards and has the following equipment

Beckman glucose analyser for whole blood glucose measurements
Beckman Coulter
Random access Hitachi Autoanalyser
Wallac Victor a multilabel counter
RIA counter
Bio Rad D10 for HbA1c measurement
PerkinElmer HPLC
Digital pH meter
Electronic balance
Refrigerated Centrifuges and high speed microcentrifuges
Freezers -80 oC (6 no.), -20 oC (6 no.)
Refrigerators (8 no.)

We are equipped to measure,

Plasma glucose, lipids, Creatinine, ALT, AST by Autoanalyser
Pancreatic hormones insulin, and, C-peptide and GAD antibody by DELFIA method
HbA1c by high performance liquid chromatography using Bio Rad D10
Microbiological assays of vitamin B12, Holo-transcobalamin and folate
Homocysteine, methionine and other sulphur compounds by HPLC
Red cell folate, Leptin and adiponectin by RIA
IGF-1, IGFBP-3, Cortisol, pro-insulin CRP, TNF-alpha, and IL-6 by ELISA
Homocysteine, Holo-transcobalamin by FPIA

The laboratory is participating in various quality control programs, namely UKEQUAS for insulin and Bio-Rad EQAS for biochemical parameters and Randox QC program for HbA1c. QC samples are also exchanged with Oxford University, Dept. of Pharmacology for vitamin B12 and folic acid. We also have our internal QC programs for all the measurements.

Laboratory personnel are trained at various international and national institutes such as Department of Pharmacology, University of Oxford, Radcliffe Infirmary, Sheffield Children's Hospital, Southampton Hospital, University of Bergen, Norway, Christian Medical College, Vellore, National Institute of Nutrition, Hyderabad, All India Institute of Medical Sciences, New Delhi, and Centre for Cellular and Molecular Biology, Hyderabad.


Techniques of Body Composition Measurements

Bioelectrical Impedance Analysis (BIA)

Bioelectrical Impedance Analysis (BIA) is a technique in which a small current is passed through the body and its resistance is measured. This resistance is used to calculate the total body water and body fat content.

Dual X-ray Absorptiometry (DXA)

We use DXA , a non-invasive procedure using very low levels of radiation to measure body fat and bone mineral density. DXA is considered to be the “gold standard” for diagnosing body fat, lean and bone mineral density.

Anthropometry

Anthropometry is a technique used to measure body size and proportions including percentage of body fat. It is commonly used techniques in epidemiology. The common measurements are – height, weight,head,chest,mid-upper arm and abdominal circumference and biceps, triceps and sub-scappular skinfolds.


Statistics And Data Management


Data Management

Data management is the most important aspect of any epidemiological research. It involves data collection, entry, verification and data retrieval when required. The main goal of data management in epidemiological research is to convert raw data from the subject into a report, in a system that is error free and easy to handle for further analysis. The investigator is responsible for collection, recording, maintenance and retrieval of data arising from the epidemiological study. All steps involved in data management should be documented properly for further auditing and performance checks.

Statistical Support

The responsibilities of statisticians in the Diabetes Unit mainly include giving advice and assistance on the statistical aspects of an experiment (Clinical / Epidemiological) and determination of sample size. Data handling, data quality checking, data description and effective presentation of statistical results are other important responsibilities. Statisticians also handle graphical presentation, analysis and interpretation of numerical data, statistical modelling and use of formal statistical arguments for determining the predictors of the several outcome variables of interest, and the use of statistical software like SPSS, STATA etc. to interpret outputs.


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